Experimental in-vitro laboratory study of the effect of acrylic removable denture base materials on the growth of representatives of the normal oral microbiota (Streptococcus salivarius)
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Methyl methacrylate at concentrations matching residual monomer levels in acrylic dentures did not significantly inhibit growth of the oral bacterium Streptococcus salivarius M18 in vitro, though a dose-dependent modulating trend was observed.
Objective. To evaluate the effect of acrylic removable denture materials on the growth of a representative of the normal oral microbiota using Streptococcus salivarius M18 as a model. Materials and methods. The study was performed in vitro. S. salivarius M18 strain was cultured in the presence of MMA at concentrations of 0.01; 0.1 and 1.0 µg/mL. The growth kinetics, biomass accumulation indicators, and bacterial cycle phase characteristics were assessed. Statistical processing was carried out using parametric and non-parametric methods at a significance level of p 0.05. Results. No statistically significant inhibitory effect of MMA on integral growth indicators was found (p 0.05). However, a dose-dependent modulating effect was observed: the low concentration (0.01 µg/mL) was accompanied by a tendency to stimulate growth, while 0.1–1.0 µg/mL was associated with a decrease in biomass accumulation in the stationary phase. The obtained data indicate the preserved viability and functional activity of the probiotic strain under conditions close to the clinical functioning of acrylic prostheses. This is of practical importance for the development of probiotic care products for prosthodontic appliances. Conclusions. Methyl methacrylate at concentrations corresponding to the residual monomer levels of acrylic dentures does not have a critical suppressive effect on the growth of S. salivarius M18. The preservation of its biological activity substantiates the possibility of using probiotic components for the prevention of microbial colonization of removable dental prostheses and reduction the risk of inflammatory complications in prosthetic dentistry.
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