Strain Profile · NCBI Species Taxon 562
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Escherichia coli DH5

DEP DH5
◔ Human-origin
Sequenced
Public genome assembly available

Escherichia coli DH5α (also written DH5) is a widely used laboratory strain engineered specifically for molecular cloning and DNA amplification; it carries genetic mutations that make it incapable of recombination and generally safe for research use, but it is not a recognized probiotic or gut health organism. The literature associated with this strain is entirely laboratory and biotechnology in nature — covering cell encapsulation for bioreactor use, transformation efficiency, and herbicide degradation studies. It has no role in human probiotic applications and is described here solely in a taxonomic and research-tool context.

Where it's been studied

Share of this strain's 3 body-system-tagged papers that concern each system. Research coverage, not measured effect.

Research

97 papers reference this strain in the probiotic database.

Match confidence
2025 IN_VITRO
Optimizing heat-shock conditions for competent cell preparation: A study on temperature and duration variability
· International Journal of Bioscience and Biochemistry
2019 OTHER
Comparación de la eficiencia de transformación entre diferentes cepas de E. coli
· DOAJ (DOAJ: Directory of Open Access Journals)
2014 OTHER
Mechanisms of Tolerance and High Degradation Capacity of the Herbicide Mesotrione by Escherichia coli Strain DH5-α
· PLoS ONE
2002 ANIMAL
[Study of alginate/chitosan microcapsules for immobilization of Escherichia coli DH5 alpha].
· PubMed
1996 ANIMAL
Microencapsulated genetically engineered E. coli DH5 cells for plasma urea and ammonia removal based on: 1. Column bioreactor and 2. Oral administration in uremic rats
· Artificial Cells Blood Substitutes and Biotechnology
2025 OTHER
A fluorescence-based protocol to monitor bacterial antagonism
· Microbiology Spectrum
2023 IN_VITRO
In vitro digestion of ESC-resistant Escherichia coli from poultry meat and evaluation of human health risk
· Frontiers in Microbiology
2014 IN_VITRO
Isolation of colicinogenic E. coli, optimization of colicin production and transformation of Col plasmid
· Journal of Applied Pharmaceutical Science
2010 IN_VITRO
A New Colored Beverage Disinfection System Using UV-A Light-Emitting Diodes
· Biocontrol Science
2026 IN_VITRO
Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain
· Global Biodiversity Information Facility
2025 IN_VITRO
Designing and construction of a single guide RNA targeted to the α-Subunit of the β-Conglycinin allergen through the CRISPR/Cas9 approach in soybean
· International Journal of Advanced Biochemistry Research
2024 ANIMAL
Molecular detection and cloning of bacterial thermophilic cellulase gene isolated from hot spring of Gilgit Baltistan
· Pure and Applied Biology
2024 IN_VITRO
Molecular Characterization of Gene Encoding Outer Membrane Protein loa22 in Pathogenic Leptospira Serovars in Iran
· Journal of Tropical Medicine
2023 OTHER
CLONING OF BRAZZEIN GENE (SWEET PROTEIN) INTO PBI121 VECTOR THROUGH PROKARYOTIC EXPRESSION SYSTEM
· The Journal of Animal and Plant Sciences
2022 OTHER
Construction of Saccharomyces cerevisiae KEX2-650 gene expression vector and its introduction into Escherichia coli DH5?
· Biodiversitas Journal of Biological Diversity
2022 OTHER
The Investigation of Clone and Expression of Butyrylcholinesterase in Pichia pastoris for Parathion Hydrolysis
· Molecular Genetics Microbiology and Virology
2021 ANIMAL
(Invited) On-Chip Characterization of Microcapsules Using a Capacitive Sensor for Microencapsulation and Single-Cell Analysis Applications
· ECS Meeting Abstracts
2021 OTHER
Membranas poliméricas para remediação ambiental : ação bactericida e remoção de corantes orgânicos e metais pesados
· LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
2019 IN_VITRO
Screening the cultivable cave microbial mats for the production of antimicrobial compounds and antibiotic resistance
· International Journal of Speleology
2018 OTHER
Expression of fluorescent tagged recombinant erythroferrone protein
· Asian Pacific Journal of Tropical Biomedicine
Browse all 97 papers →

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