Clostridium thermocellum

Clostridium thermocellum, a promising candidate for consolidated bioprocessing, has been subjected to numerous engineering strategies for enhanced bioethanol production. Measurements of intracellular metabolites at substrate concentrations high enough (.50 g/L) to allow the production of industrially relevant titers of ethanol would inform efforts toward this end but have been difficult due to the production of a viscous substance that interferes with the filtration and quenching steps during metabolite extraction. To determine whether this problem is unique to C. thermocellum, we performed filtration experiments with other organisms that have been engineered for high-titer ethanol production, including Escherichia coli and Thermoanaerobacterium saccharolyticum. We addressed the problem through a series of improvements, including active pH control (to reduce problems with viscosity), investigation of different filter materials and pore sizes (to increase the filtration capacity), and correction for extracellular metabolite concentrations, and we developed a technique for more accurate intracellular metabolite measurements at elevated substrate concentrations.

Reporter Group-Labeled Synthetic Cellulose: Structural Characterization and Utilization in Mapping the Cellulose Chain-Cleavage Modes of Cellulases

Visual exoproteomics of Clostridium thermocellum during anaerobic biomass-degradation identifies functional spirosomes

Waste-to-energy approach by transforming municipal solid waste into biohydrogen and biomethane through dark fermentation with the yield of biofertilizer

Genetic and metabolic drivers of membrane remodeling in Clostridium thermocellum under alcohol stress

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose‐Degrading Anaerobic Bacteria

Genome-based Study on the mechanism of rare earth neodymium ions increasing ethanol production from Clostridium thermocellum

Recombineering machinery to increase homology directed genome editing in thermophilic microbes

The final proteolytic step in transmembrane signaling of multiple RsgI anti-σ factors in Clostridium thermocellum

Optimization of biohydrogen production by modifying metabolic pathways in Clostridium Thermocellum

Semi-continuous fermentation of unpretreated corn stover in a novel high solids bioreactor by a coculture of Clostridium thermocellum and Thermoanaerobacterium thermosaccharolyticum

Thermodynamics shapes the in vivo enzyme burden of glycolytic pathways

Microbial-photoelectrochemical cell for the conversion of raw cellulose materials into electrical power and chemicals

Thermodynamics shape the in vivo enzyme burden of glycolytic pathways

Positive effects of in-situ composting of tea plant pruning residues on soil fertility, tea quality, and microbial diversity

Optimized whole-cell depolymerization of polyethylene terephthalate to monomers using engineered Clostridium thermocellum

Deconstruction by C. thermocellum—from microbe mediated to dynamic redistribution of cellulosomes

EVALUACIÓN DE UN SISTEMA DE CULTIVO DE CLOSTRIDIUM THERMOCELLUM PARA LA PRODUCCIÓN DE BIOETANOL

Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates

A cellulosomal double‐dockerin module from Clostridium thermocellum shows distinct structural and cohesin‐binding features