Strain Profile
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Bacillus subtilis

◑ Environmental
Sequenced
Public genome assembly available

Bacillus subtilis is a Gram-positive, rod-shaped bacterium widely distributed in soil and food environments, including fermented products. Animal studies have examined the strain's potential effects on digestive health and immune function. In vitro research has investigated its biofilm formation and metabolic capabilities. Human clinical evidence is limited, and strain-specific efficacy remains to be established through randomized controlled trials.

Where it's been studied

Share of this strain's 585 body-system-tagged papers that concern each system. Research coverage, not measured effect.

+6 more systems

Symptoms in the research

Share of this strain's 99 symptom-tagged papers that mention each symptom or condition. Research coverage, not evidence of benefit.

+13 more symptoms

Strains of this species

28 strains of Bacillus subtilis in the index, with 869 linked papers between them.

Bacillus subtilis DE111

™ DE111

Bacillus subtilis strain DE111 is a gram-positive spore-forming bacterium marketed as a probiotic supplement.

Barrier AMR & toxins Immune
◑ Environmental
21 papers 0 comments

Bacillus subtilis HU58

™ HU58

Bacillus subtilis HU58 is a spore-forming probiotic strain with a relatively well-developed human clinical evidence base compared to many other Bacillus strains.

Barrier Metabolic Immune
◑ Environmental
19 papers 0 comments

Bacillus subtilis DCP04

DEP DCP04

Bacillus subtilis DCP04 is a strain isolated from doenjang, a traditional Korean fermented soybean paste.

Adhesion Microplastics AMR & toxins
◑ Environmental
1 papers 0 comments

Bacillus subtilis NPA6

DEP NPA6

Bacillus subtilis NPA6 is a strain of B. subtilis, a spore-forming species with a long history of use in fermented foods and agricultural applications.

◑ Environmental
1 papers 0 comments

Bacillus subtilis RBM2

DEP RBM2

Bacillus subtilis RBM2 appears in the literature primarily in the context of environmental plastic biodegradation rather than human probiotic research.

Microplastics
◑ Environmental
3 papers 0 comments

Bacillus subtilis ATCC 6633

ATCC 6633

Bacillus subtilis ATCC 6633 is a spore-forming aerobic bacterium widely used in research and biotechnology.

Survival Metabolic AMR & toxins
◑ Environmental
785 papers 0 comments

Bacillus subtilis F6

DEP F6

Bacillus subtilis F6 is a Bacillus subtilis strain for which no strain-specific primary or secondary research papers were identified in the indexed literature.

◑ Environmental
0 papers 0 comments

Bacillus subtilis W7

DEP W7

Bacillus subtilis W7 is a spore-forming bacterium belonging to a species with a long history of use in fermented foods and agricultural biocontrol.

◑ Environmental
2 papers 0 comments

Bacillus subtilis P22

DEP P22

Bacillus subtilis P22 is a strain designation within B.

◑ Environmental
1 papers 0 comments

Bacillus subtilis ACT-8

DEP ACT-8

Bacillus subtilis ACT-8 is a strain for which no indexed research papers were identified in the current evidence base.

◑ Environmental
1 papers 0 comments

Bacillus subtilis CSM5

DEP CSM5

Bacillus subtilis CSM5 is a designated strain of B.

◑ Environmental
1 papers 0 comments

Bacillus subtilis P165

DEP P165

Bacillus subtilis P165 is a designated strain within the well-studied B.

◑ Environmental
1 papers 0 comments

Bacillus subtilis ATCC 21332

ATCC 21332

Bacillus subtilis ATCC 21332 is a strain studied primarily for its production of surfactin, a lipopeptide (fat-linked peptide) compound with antimicrobial and biosurfactant properties.

Metabolic Precision Adhesion
◑ Environmental
13 papers 0 comments

Bacillus subtilis N6

DEP N6

Bacillus subtilis N6 appears in the indexed literature in a single 2026 in vitro study examining polyester fiber degradation, an environmental biotechnology application with no relation to human health or probiotic suppl…

Metabolic
◑ Environmental
1 papers 0 comments

Bacillus subtilis ZE15

DEP ZE15

Bacillus subtilis strain ZE15 is a gram-positive soil bacterium.

Adhesion Metabolic
◑ Environmental
1 papers 0 comments

Bacillus subtilis ZE16

DEP ZE16

Bacillus subtilis ZE16 is a strain for which no primary or secondary relevance papers were identified in the current research index.

◑ Environmental
0 papers 0 comments

Bacillus subtilis ZE12

DEP ZE12

Bacillus subtilis ZE12 is a named strain of the widely studied Bacillus subtilis species.

◑ Environmental
0 papers 0 comments

Bacillus subtilis BPM12

DEP BPM12

Bacillus subtilis BPM12 has been studied in a single 2023 in vitro publication examining its ability to hydrolyze BHET (bis(2-hydroxyethyl) terephthalate), a breakdown product of polyethylene terephthalate (PET) plastic,…

Metabolic Microplastics Survival
◑ Environmental
1 papers 0 comments

Bacillus subtilis BI8

DEP BI8

Bacillus subtilis BI8 appears in this index in the context of environmental microbiology research.

Adhesion Metabolic Microplastics
◑ Environmental
1 papers 0 comments

Bacillus subtilis B26

DEP B26

Bacillus subtilis B26 is a plant-associated strain characterized primarily in agricultural research for its ability to colonize grass and crop roots, promote plant growth, and confer drought tolerance.

Survival Adhesion Metabolic
◑ Environmental
6 papers 0 comments

Bacillus subtilis A4

DEP A4

Bacillus subtilis A4 is a spore-forming bacterium belonging to a species with broad applications in fermented foods (notably natto in Japan and Africa's dawadawa), industrial enzyme production, and agricultural biocontro…

◑ Environmental
1 papers 0 comments

Bacillus subtilis FW1

DEP FW1

Bacillus subtilis FW1 is a named isolate of Bacillus subtilis with no strain-specific published evidence in the current indexed literature for probiotic or human health applications.

◑ Environmental
3 papers 0 comments

Bacillus subtilis P74 I

P74 I

Bacillus subtilis P74-I is a designated strain of B.

◑ Environmental
0 papers 0 comments

Bacillus subtilis MTCC 144

MTCC 144

Bacillus subtilis MTCC 144 is an Indian culture collection deposit within the B.

◑ Environmental
1 papers 0 comments

Research

1342 papers reference this strain in the probiotic database.

Match confidence
2025 IN_VITRO
Enhancement of gut barrier integrity by a Bacillus subtilis secreted metabolite through the GADD45A‐Wnt/β‐catenin pathway
· iMeta
2025 IN_VITRO
Engineered bacillus subtilis enhances bone regeneration via immunoregulation and anti-Infection
· Bioactive Materials
2025 ANIMAL
Bacillus subtilis HGCC-1 improves growth performance and liver health via regulating gut microbiota in golden pompano
· Animal Microbiome
2025 IN_VITRO
Surfactin facilitates establishment of Bacillus subtilis in synthetic communities
· The ISME Journal
2024 IN_VITRO
Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth
· Physiologia Plantarum
2024 IN_VITRO
Role of Bacillus subtilis exopolymeric genes in modulating rhizosphere microbiome assembly
· Environmental Microbiome
2024 IN_VITRO
Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis
· Biotechnology and Applied Biochemistry
2024 IN_VITRO
Probiotic Bacillus subtilis SB8 and edible coatings for sustainable fungal disease management in strawberry
· Biological Control
2024 IN_VITRO
Immunomodulatory properties of Bacillus subtilis extracellular vesicles on rainbow trout intestinal cells and splenic leukocytes
· Frontiers in Immunology
2024 OBSERVATIONAL
Effects of Trichoderma harzianum and Bacillus subtilis on the root and soil microbiomes of the soybean plant INTACTA RR2 PRO™
· Frontiers in Plant Science
2024 REVIEW
Spore-Based Probiotic Bacillus subtilis: Current Applications in Humans and Future Perspectives
· Fermentation
2024 IN_VITRO
Bioturbation analysis of microbial communities and flavor metabolism in a high-yielding cellulase Bacillus subtilis biofortified Daqu
· Food Chemistry X
2024 ANIMAL
Impact of Bacillus subtilis Probiotic on Growth Performance, Bone Health, IntestinalMorphology, and Cecal Microbiota in Cobb Broiler Chicks
· Pakistan Veterinary Journal
2024 ANIMAL
Effects of Bacillus subtilis as a single strain probiotic on growth, disease resistance and immune response of striped catfish (Pangasius hypophthalmus)
· PLoS ONE
2024 OTHER
Competition for iron shapes metabolic antagonism between Bacillus subtilis and Pseudomonas marginalis
· The ISME Journal
2024 IN_VITRO
Bacillus subtilis-derived peptides disrupt quorum sensing and biofilm assembly in multidrug-resistant Staphylococcus aureus
· mSystems
2024 OTHER
Mechanisms and strategies for regulating sporulation and germination of Bacillus subtilis to enhance probiotic effects
· Industrial Crops and Products
2024 OTHER
Important role of Bacillus subtilis as a probiotic and vaccine carrier in animal health maintenance
· World Journal of Microbiology and Biotechnology
2024 ANIMAL
Bacillus subtilis improves antioxidant capacity and optimizes inflammatory state in broilers
· Animal Bioscience
2024 IN_VITRO
Effects of Bacillus subtilis on in vitro ruminal fermentation and methane production
· Translational Animal Science
Browse all 1,342 papers →

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Citations

Selected papers that name this strain.

  1. [1]
    Spore-Based Probiotic Bacillus subtilis: Current Applications in Humans and Future Perspectives
    · Fermentation · 2024
    Source →
  2. [2]
    Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō
    · Frontiers in Microbiology · 2023
    PMID 37840737 →
  3. [3]
    Mechanism of TLR4-Mediated Anti-Inflammatory Response Induced by Exopolysaccharide from the Probiotic Bacillus subtilis
    · The Journal of Immunology · 2023
    PMID 37672039 →
  4. [4]
    Feeding with 4,4′-diaponeurosporene-producing Bacillus subtilis enhances the lactogenic immunity of sow
    · BMC Veterinary Research · 2023
    PMID 38115003 →
  5. [5]
    Safety and Immunogenicity of Inactivated Bacillus subtilis Spores as a Heterologous Antibody Booster for COVID-19 Vaccines
    · Vaccines · 2022
    PMID 35891178 →
  6. [6]
    Bacillus subtilis chassis in biomanufacturing 4.0
    · Journal of Chemical Technology & Biotechnology · 2022
    Source →
  7. [7]
    The probiotic Bacillus subtilis BS50 decreases gastrointestinal symptoms in healthy adults: a randomized, double-blind, placebo-controlled trial
    · Gut Microbes · 2022
    PMID 36269141 →
  8. [8]
    Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract: A Randomized, Crossover, Double-Blind, and Placebo-Controlled Study
    · Frontiers in Microbiology · 2021
    PMID 34408741 →
  9. [9]
    Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
    · International Journal of Molecular Sciences · 2021
    PMID 33671071 →
  10. [10]
    Safety Assessment of Bacillus subtilis MB40 for Use in Foods and Dietary Supplements
    · Nutrients · 2021
    PMID 33668992 →